203 - The Potential for Bone Marrow Mesenchymal Stem Cells Slowing Nucleus P...

#203 The Potential for Bone Marrow Mesenchymal Stem Cells Slowing Nucleus Pulposus Cells Degeneration through NF-ĸB Pathway

General Session: Best Papers Session 2

Presented by: J. Zou

Author(s):

J. Zou (1)

(1) The First Affiliated Hospital of Soochow University, Suzhou, China

Abstract

Introduction: Lumbar disc degeneration is a common cause of low back pain. Current treatment strategies, including conservative and surgical treatment are aimed only at alleviating symptoms, and often lead to recurrence of in situ or adjacent disc disease. Consequently, searching for more effective treatments for disc degeneration has been a research hotspot by for now. The objective was to explore the mechanism of bone marrow mesenchymal stem cells (BMSCs) slow nucleus pulposus cells (NPCs) degeneration.

Methods: Human bone marrow mesenchymal stem cells and lumbar nucleus pulposus cells were harvested and co-cultured. The experiment was divided into three groups: pure NPCs culture group (Group A), pure BMSCs culture group (Group B), NPCs and BMSCs (1:1) co-culture group (Group C). CCK8 determined the cell proliferation of the three groups of 3, 5 and 7 days. mRNA expression of Collagen II and Aggrecan were detected by PCR, TGF-βl levels were measured using ELISA, content of NF- ĸB was detected by Western-blot methods, and Collagen II expression was assayed via immunohistochemical staining among groups at each time point.

Results: The ability of cell proliferation of group C was greater than the other groups after 3 days. The expression of Aggrecan and Collagen II of group C was higher than the other groups after 7 days. The content of TGF-βl of group C was higher than the other groups, and the content of NF-ĸB was lower after 7 days. Immunohistochemical staining of Collagen II in group C showed strong positive expression on the 7th day.

Conclusion: BMSCs would promote proliferation and the expression of Aggrecan, Collagen II and TGF-βl of NPCs. BMSCs delaying NPCs degeneration through inhibiting the NF-ĸB pathway via up-regulated TGF-β1.